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blasticidin  (Thermo Fisher)


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Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
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    Structured Review

    Thermo Fisher blasticidin
    Blasticidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blasticidin/Blasticidin+S+hydrochloride%2C+98%2B%25%2C+in+1M+HEPES+buffer+soln/pm42259351-86-15-21
    Average 97 stars, based on 1 article reviews
    blasticidin - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Genomic landscape of drug binding and pharmacogenetic variation across diverse populations using SNPdrug3D.
    Article Snippet: .. HEK293 Flp-In, T-ReX cell line was purchased from Invitrogen (#R78007) and cultured in DMEM containing 10% FBS (Excell), 1% NEAA, 1% Penicillin/Streptomycin (Gibco), 15g/ml Blasticidin (Invitrogen) and 100g/ml Zeocin (Invitrogen). ..

    Transduction:

    Article Title: Inflammatory cytokines induce new cancer dependencies.
    Article Snippet: Collins K. Cheruiyot, Sarah Y. Kim, Juan Dubrot , Sarah K. Lane-Reticker, Alex Miranda, Ashwin V. Kammula , Jonathan J. Perera , Peter P. Du , Cun Lan Chuong, Rachel A. Fetterman, Nelson H. Knudsen , Aiping Jiang , Juliette S. M. T. Suermondt, Lomax F. Pass, Cong Fu, Meng-Ju Wu , Lei Shi , Seth Anderson , Audrey J. Muscato , Omar I. Avila , Ian C. Kohnle, Emily A. Kessler , Hans W. Pope, Sarah G. Noel , Kira E. Olander , Jooho Chung , Kayla J. Colvin, Nabeel Bardeesy, Kathleen B. Yates & Robert T. Manguso

    Stable Transfection:

    Article Title: Widespread circulation of Alongshan virus in Austria and serological evidence for infection in humans: a nationwide molecular and serological observational study.
    Article Snippet: Stable S2 cell lines were established by calcium phosphate transfection with the respective expression plasmid and a blasticidin resistance plasmid, following the manufacturer’s protocol (Invitrogen Calcium Phosphate Transfection Kit, Thermo Fisher Scientific). .. Stably transfected S2 cells were then selected by supplementing the culture medium with 25 μg/mL blasticidin (Blasticidin S HCl, 100 mg; Thermo Fisher Scientific). .. For protein production, transfected cells were adapted to serum-free medium (Insect-XPRESS Protein-free Insect Cell Medium, Lonza, Basel, Switzerland) containing 10 μg/mL blasticidin and cultivated at 28◦C under shaking conditions.

    Transfection:

    Article Title: Widespread circulation of Alongshan virus in Austria and serological evidence for infection in humans: a nationwide molecular and serological observational study.
    Article Snippet: Stable S2 cell lines were established by calcium phosphate transfection with the respective expression plasmid and a blasticidin resistance plasmid, following the manufacturer’s protocol (Invitrogen Calcium Phosphate Transfection Kit, Thermo Fisher Scientific). .. Stably transfected S2 cells were then selected by supplementing the culture medium with 25 μg/mL blasticidin (Blasticidin S HCl, 100 mg; Thermo Fisher Scientific). .. For protein production, transfected cells were adapted to serum-free medium (Insect-XPRESS Protein-free Insect Cell Medium, Lonza, Basel, Switzerland) containing 10 μg/mL blasticidin and cultivated at 28◦C under shaking conditions.

    Expressing:

    Article Title: Presentation of identical tumor antigen peptides by canine and human MHC class I
    Article Snippet: .. Cells were selected using 10 μg/ml of Blasticidin (ThermoFisher) and then the cell surface expression of DLA molecules were verified using flow cytometry using H58A and BB7.6 antibodies. .. To establish a clonal population of cells expressing monoallelic DLA molecules, cells went through a final round of bulk sorting after staining with H58A or BB7.6 (BD FACSCalibur, Becton Dickinson) ( C–1E).

    Article Title: A conserved mechanism for regulation of mtDNA copy number in eukaryotes
    Article Snippet: Viral supernatant was filter sterilised and added to SLC25A39KO3 and the control clone cells for 48 hours after addition of 6μg/mL polybrene (Merck, TR-1003). .. Cells with integrated plasmid were selected using 10ug/ml blasticidin (Life Technologies) and bulk populations were characterised by western blot after doxycycline induced expression of SLC25A39wt or SLC25A39Δ72-86. .. Mito-tag U2OS cells were generated using virus produced in Phoenix-ampho cells following transfection with pMXs-IRES-Bla-3HA-EGFP-OMP25 (David Sabatini, Addgene Plasmid #83356) using Lipofectamine 2000 according to the manufacturer’s instructions and as above.

    Flow Cytometry:

    Article Title: Presentation of identical tumor antigen peptides by canine and human MHC class I
    Article Snippet: .. Cells were selected using 10 μg/ml of Blasticidin (ThermoFisher) and then the cell surface expression of DLA molecules were verified using flow cytometry using H58A and BB7.6 antibodies. .. To establish a clonal population of cells expressing monoallelic DLA molecules, cells went through a final round of bulk sorting after staining with H58A or BB7.6 (BD FACSCalibur, Becton Dickinson) ( C–1E).

    Infection:

    Article Title: Isolation of adipose-derived mesenchymal stem cells expressing soluble forms of GAS1 and PTEN for experimental cell therapy for glioblastoma
    Article Snippet: Glioblastoma is the most frequent primary brain tumor, and its current treatment mainly prolongs survival, highlighting the need for more effective second-line therapies to improve patient prognosis.. Stem cells represent a promising platform for developing cell-based therapies due to their biological characteristics, which enable the delivery of antitumoral agents.. Still, there are some limitations, such as invasive delivery methods to overcome the blood-brain barrier, and the need for repeated administration, among others.

    Incubation:

    Article Title: Isolation of adipose-derived mesenchymal stem cells expressing soluble forms of GAS1 and PTEN for experimental cell therapy for glioblastoma
    Article Snippet: Glioblastoma is the most frequent primary brain tumor, and its current treatment mainly prolongs survival, highlighting the need for more effective second-line therapies to improve patient prognosis.. Stem cells represent a promising platform for developing cell-based therapies due to their biological characteristics, which enable the delivery of antitumoral agents.. Still, there are some limitations, such as invasive delivery methods to overcome the blood-brain barrier, and the need for repeated administration, among others.

    Selection:

    Article Title: Isolation of adipose-derived mesenchymal stem cells expressing soluble forms of GAS1 and PTEN for experimental cell therapy for glioblastoma
    Article Snippet: Glioblastoma is the most frequent primary brain tumor, and its current treatment mainly prolongs survival, highlighting the need for more effective second-line therapies to improve patient prognosis.. Stem cells represent a promising platform for developing cell-based therapies due to their biological characteristics, which enable the delivery of antitumoral agents.. Still, there are some limitations, such as invasive delivery methods to overcome the blood-brain barrier, and the need for repeated administration, among others.

    Plasmid Preparation:

    Article Title: A conserved mechanism for regulation of mtDNA copy number in eukaryotes
    Article Snippet: Viral supernatant was filter sterilised and added to SLC25A39KO3 and the control clone cells for 48 hours after addition of 6μg/mL polybrene (Merck, TR-1003). .. Cells with integrated plasmid were selected using 10ug/ml blasticidin (Life Technologies) and bulk populations were characterised by western blot after doxycycline induced expression of SLC25A39wt or SLC25A39Δ72-86. .. Mito-tag U2OS cells were generated using virus produced in Phoenix-ampho cells following transfection with pMXs-IRES-Bla-3HA-EGFP-OMP25 (David Sabatini, Addgene Plasmid #83356) using Lipofectamine 2000 according to the manufacturer’s instructions and as above.

    Western Blot:

    Article Title: A conserved mechanism for regulation of mtDNA copy number in eukaryotes
    Article Snippet: Viral supernatant was filter sterilised and added to SLC25A39KO3 and the control clone cells for 48 hours after addition of 6μg/mL polybrene (Merck, TR-1003). .. Cells with integrated plasmid were selected using 10ug/ml blasticidin (Life Technologies) and bulk populations were characterised by western blot after doxycycline induced expression of SLC25A39wt or SLC25A39Δ72-86. .. Mito-tag U2OS cells were generated using virus produced in Phoenix-ampho cells following transfection with pMXs-IRES-Bla-3HA-EGFP-OMP25 (David Sabatini, Addgene Plasmid #83356) using Lipofectamine 2000 according to the manufacturer’s instructions and as above.



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